Viabilitas Azotobacter A1a, A3, dan A9 pada Medium yang Terpapar Logam Cadmium (Cd)

Denaya Andrya Prasidya, Enny Zulaika
Submission Date: 2015-08-24 23:00:36
Accepted Date: 2016-01-21 09:28:22


Kadmium adalah salah satu logam berat yang beracun walaupun dalam konsentrasi yang rendah. Beberapa bakteri genus Azotobacter resisten terhadap logam kadmium. Tujuan dari penelitian ini adalah mendapatkan genus Azotobacter yang resisten terhadap CdCl2. Uji resistensi menggunakan medium Nutrient Agar (NA) yang mengandung CdCl2 10-50 mg/L. Uji viabilitas Azotobacter dilakukan pada 3 isolat yang dipilih dari uji resistensi. Isolat tersebut dikategorikan lebih resisten dari isolat yang lain. Uji viabilitas dilakukan pada medium Nutrient Broth (NB) yang mengandung CdCl210, 20, dan 30 mg/L. Kepadatan sel diukur dengan Spektrofotometer UV-Vis pada panjang gelombang 600 nm selama 24 jam. Hasil penelitian menunjukkan isolat Azotobacter A1a, A1b, A2, A3, A5, A6, A7, A8, A9, A10 resisten pada medium Nutrient Agar (NA) yang mengandung CdCl2 sampai dengan konsentrasi 50 mg/L. Kurva pertumbuhan isolat Azotobacter yang tercekam CdCl2relatif lebih rendah dibandingkan isolat Azotobacter yang tidak tercekam CdCl2.


Azotobacter; kadmium; konsentrasi; resistensi.


J.A. Almeida, R.E. Barreto, L.B. Novelli, F.J. Castro, and S.E. Moron, “Oxidative Stress Biomarkers and Aggressive Behavior in Fish Exposed to Aquatic Cadmium Contamination,” Neotropical Ichtyology, Vol. 7 (2009) 103-108.

S.H. Chien, G. Carmona, L.L. Prochnow, and E.R. Austin, “Cadmium Avalability From Granulated and Bulk-blended Phosphate-potassium Fertilizers,” Journal Environ. Qual 32 (2003) 1911-1914.

M. Maanan, “Biomonitoring of Heavy Metals Using Mytilus galloprovincialis in Safi Coastal Waters, Morocco,” Environmental Toxicology, Vol. 22, Issue 5 (2007) 525-531.

L. Patrick, “Toxic Metals and Antioxidants Part II the Role of Antioxidant Ni Arsenic and Cadmium Toxicity – Toxic Metals part II,” Review, Alternativer Medicine (2003).

S.J.S. Flora, “Metal Poisoning: Treatment and Management,” Review Article, Al Ameen Journal Medicine Science, Vol. 2 (2009) 4-26.

J. Gzy, K. Rymer, and E.A. Gwozdz,” Differential Response of Antioxidant Enzymes to Cadmium Stress in Tolerant and Sensitive Cell Line of Cucumber (Cucumis sativus L), Acta Biochemica Polonica Vol. 56 (2009) 723-727.

R. John, P. Ahmad, K. Gadgila, and S. Sharmab, “Heavy Metal Toxicity: Effect on Plant Growth, Biochemical Parameters and Metal Accumulation by Brassica juncea L.,” International Journal of Plant Production, Vol. 3 (2009) 65-76.

M.R. Bruins, S. Kapil, and F.W. Oehme, “Microbial Resistance to Metals in the Environment (Review),” Exotoxicology and Environmental Safety Vol. 45 (2000) 198-207.

D.H. Nies, “Efflux- mediated Heavy Metal Resistance in Prokaryotes, “ Federation of European Microbiological Societes, Microbiology Reviews 27 (2003) 313-339.

S.E. Elsilk, A.E.R. El-Shanshoury, and P.S. Ateya, “Accumulation of some heavy metals by metal resistant avirulent Bacillus anthracis PS2010 isolated from Egypt,” African Journal of Microbiology Research Vol 8 (2014) 1266-1276.

A. Luqman,, M. Shovitri, dan E. Zulaika, “Resistensi Bakteri Azotobacter Terhadap Logam Berat. Seminar Nasional Scientific Conference of Environmetal Technology IX “Advances in Agricultural and Municipal Waste Technology to Anticipate Food and Energy Crisis, July 10 (2012). Teknik Lingkungan, FTSP-ITS Surabaya Indonesia.

A.E. Abo-amer, M.A. Abu-gharbia, E.M. Soltana, and W.M. Abd El-Raheem, “Isolation and Molecular Characterization of Heavy Metal-Resistant Azotobacter chroococcum from Agricultural Soil and their Potential Application in Bioremediation,” Geomicrobiology Journal Vol. 31(2014) 551–561.

P.M. Joshi, dan A.A. Juwarkar, “In Vivo Studies To Elucidate The Role Of Extracellular Polymeric Substances From Azotobacter In Immobilization Of Heavy Metals,” Environmental Science & Technology Vol. 43, No. 15 (2009) 5884–5889.

K. Khotimah, dan E. Zulaika, “Azotobacter sebagai Bioakumulator Merkuri,” Jurnal Sains Pomits. Vol. 2 (2013).

H. Abd-Elnaby, G.M. Abou-Elela, and N.A. El-Sersy, “Cadmium resisting Bacteria in Alexandria Eastern Harbor (Egypt) and Optimization of Cadmium Bioaccumulation by Vibrio harveyi,” African Journal of Biotechnology Vol. 10, No. 17 (2011) 3412-3423.

T.I. Stuczynski, G.W. McCarty, and G. Siebielec, “Response of Soil Microbiological Activities to Cadmium, Lead, and Zinc Salt Amendments. Journal Environ. Qual Vol. 32 (2003) 1346-1355.

D.H. Nies, “Microbial Heavy Metal Resistance: Molecular Biology and Utilisation for Biotechnological Processes,” Appl. Microbiol Biotechnol Vol. 51 (1999) 730-750.

B.A. Rasulov, A. Yili, and H.A. Aisa, “Biosorption of Metal Ions by Exopolysaccharide Produced by Azotobacter chroococcum XU1. Journal of Environmental Protection (2013) 989-993.

S.A.F.T. van Hijum, GS van, H. Rahaoui, D.M. van, and L. Dijkhuizen, “Characterization of a novel fructosiltransferase from Lactobacillus reuteri that synthesizes high-molecular-weight inulin and Inulin oligosaccharides,” Appl. Environ. Microbiol Vol. 68 No. 9 (2002) 4390- 4398.

R. Hindersah, D.H. Arief, S. Soemitro, dan L. Gunarto, ”Pengaruh CdCl2 terhadap Produksi Eksopolisakarida dan Daya Hidup Azotobacter,” Jurnal Natur Indonesia Vol. 12 No. 1 (2009) 34-37.

M.T. Madigan and J.M. Martinko, “Brock Biology of Microbiology. Prentice-Hall” (2000).

M.D. Rolfe, C.J. Rice, S. Lucchini, C. Pin, A. Thompson, A.D.S. Cameron, M. Alston, M.F. Stringer, R.P. Betts, J. Baranyi, M.W. Peck, and J.C.D. Hintona, “Lag Phase is a Distinct Growth Phase That Prepares Bacteria for Exponential Growth and Involves Transient Metal Accumulation,” Journal of Bacteriology (2012) 686–701.

H. Abd-Elnaby, G.M. Abou-Elela, and N.A. El-Sersy, “Cadmium resisting Bacteria in Alexandria Eastern Harbor (Egypt) and Optimization of Cadmium Bioaccumulation by Vibrio harveyi,” African Journal of Biotechnology Vol. 10, No. 17 (2011) 3412-3423.

R. Choudhury, and S. Srivastava, “Zinc Resistance Mechanisms in Bacteria,” Review Article Current Science Vol 81 No. 7 (2001) 768-775.

G. Emtiazi, Z. Ethemadifar, and M.H. Habibi, “Production of Extracellular Polymer in Azotobacter and Biosorption of Metal by Exopolymer,” Africa Journal Biotechnology Volume 3 (2004) 330-333.

C. Li, and H.H.P. Fang, “Fermentative Hydrogen Production from Waste Water and Solid Wastes by Mixed Cultures Cr,” Rev Environ Sciences Technology Volume 37 No.1 (2007) 31-39.

N. Hidayah, dan M. Shovitri, “Adaptasi Isolat Bakteri Aerob Penghasil Gas Hidrogen pada Medium Limbah Organik. Jurnal Sains dan Seni ITS Volume 1 (2012).

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