Peningkatan Masa Simpan Aktivator Kompos melalui Variasi Sumber Nitrogen

Arida Wahyu Barselia, Dr.techn.Endry Nugroho Prasetyo,MT, Endry Nugroho Prasetyo
Submission Date: 2016-01-26 20:15:45
Accepted Date: 2016-04-28 10:46:38

Abstract


Kompos merupakan hasil degradasi bahan organik secara aerob maupun anaerob dengan memanfaatkan potensi mikroorganisme sebagai aktivator kompos. Kualitas aktivator kompos sangat tergantung dari kecepatan degradasi yang dimiliki dan lama masa simpannya. Medium pertumbuhan berpengaruh penting terhadap masa simpan aktivator kompos karena untuk mengomptimalkan viabilitas sel dalam waktu yang lama.

Salah satu komponen medium pertumbuhan adalah nitrogen berupa protein atau senyawa peptida yang dapat memengaruhi masa simpan (life span) aktivator kompos. Kompleksitas struktur protein menyebabkan pemanfaatan nitrogen untuk pertumbuhan juga berbeda, yang salah satunya berpengaruh terhadap masa simpan aktivator kompos.

Tujuan penelitian ini adalah mengetahui pengaruh beberapa sumber nitrogen antara lain yeast extract, pepton, susu skim, dan rumput laut, yang berpotensi untuk meningkatan masa simpan aktivator kompos.

Konsorsium aktivator kompos berupa Aspergillus niger dan Rhizobium sp. dengan perbandingan 1:1 dikultur pada medium variasi sumber nitrogen dengan konsentrasi yang berbeda yaitu 2,5% dan 1,25% dengan 3 kali pengulangan. Jumlah koloni dihitung dengan metode TPC (Total Plate Agar), Kadar protein terlarut pada medium kultur dianalisis dengan metode Bradford. Hasil kultur aktivator kompos diaplikasikan untuk pembuatan kompos. Data dianalisis secara deskriptif berupa kurva pertumbuhan koloni, kemiringan kurva pertumbuhan aktivator kompos,kadar protein terlarut, suhu kompos, dan tabel analisis rasio C/N. Hasil penelitian didapatkan bahwa medium rumput laut konsentrasi 1,25% mampu meningkatkan masa simpan aktivator kompos karena hasil kemiringan kurva pertumbuhan relatif rendah sehingga diasumsikan jumlah sel yang cenderung stabil. Sedangkan nilai rasio C/N terbaik adalah aktivator kompos pada medium rumput laut konsentrasi 1,25% karena nilai yang didapatkan sebesar 14,15/1.Sehingga medium rumput laut konsentrasi 1,25% dapat dimanfaatkan untuk meningkatkan masa simpan aktivator kompos.


Keywords


aktivator kompos; masa simpan; variasi dumber nitrogen.

References


J. A. Alburquerque, J. G. lvez, G. Tortosa, G. Baddi, J. Cegarra, “Evaluation of ‘‘alperujo’’ composting based on organic matter degradation, humification and compost quality”. Journal of Biodegradation Springer Science and Business Media (2009) 20:257–270.

O. Fourti, N.Jedidi, dan A. Hassen. “Humic substances change during the co-composting process of municipal solid wastes and sewage sludge”. Journal of Microbiol Biotechnol (2010) 26:2117–2122.

A.Kumar, S. Gaind, dan, L. Nain. :Evaluation of thermophilic fungal consortium for paddy straw composting”. Journal of Biodegradation (2008) 19:395–402.

R. Li, L. Li, R. Huang, Y. Su, X. Mei, B. Shen, dan Q. Shen. “Variations of culturable thermophilic microbe numbers and bacterial communities during the thermophilic phase of composting”. Journal of World Microbiol Biotechnol (2013) 30:1737–1746.

I. Petric, dan V. Selimbas. “Composting of poultry manure and wheat straw in a closed reactor: optimum mixture ratio and evolution of parameters”. Journal of Biodegradation (2007)19:53–63.

Gharib, F . A., M Oussa, L. A. , Dan Massoud, Osama. N. “Effect of compost and bio-fertilizers on growth, yield and essential oil of sweet marjoram (Majorana hortensis) plant”. International journal Of Agriculture & Biology (2008) 1560–8530.

Partanen, P., Hultman, J., Paulin, L., Auvinen, P. , dan Romantschuk, M. “Bacterial diversity at different stages of the composting process”. Research Article BMC Microbiology (2010) 10-94 [25 Agustus 2015].

Skinner, C. dan Lin, S. J. “Effects of calorie restriction on life span of microorganisms”. Journal of Appl Microbiol Biotechnol (2010) 88:817–828.

Delalibera, I. Jr., Humbler, R. A., Hajek, A. E. “Preservation of in vitro culture of the mite pathogenis fungus Neozygites tanajoae”. Canadian Journal of Microbiology 8:50 page (2004 )579.

Romano, P.. “Laboratory procedures for microorganisms”. Artikel CABRI. (1998) April 2013.

Uzunova, T. D., dan Donev, T. “Anabiosis and conservation of microorganisms”. Journal Of Culture Collections Volume 4, (2005 ) 17-28.

Mukherjee,T. K., Raghavan, A., dan Chatterji, D. “Shortage of nutrient in bacteria: The Stringent Response”. Review Article Current Science (1998) Volume 75, No. 7.

Hubalek, Z. “Protectants used in the cryopreservation of microorganisms”. Review Journal Cryobiology 46 (2003) 205-229.

Ooi, T. C., 1 Ariff, A. B., Halimi, M. S., dan Shamsuddin, Z. H. “Growth kinetics of diazotrophic Bacillus sphaericus UPMB 10 cultured usingdifferent types and concentrations of carbon and nitrogen sources”. Malaysian Journal of Microbiology, Vol 4 (2008) 15- 25.

Uzeh, R. E., Akinola, S. O., dan and Olatope, S. O. A. " Production of peptone from soya beans (Glycine max L merr) and African locust beans (Parkia biglobosa)”. African Journal of Biotechnology Vol. 5 (18), (2006) 1684-1686.

Ravimannan, N., Arulanantham, R., Pathmanathan, S., dan Niranjan, K. “Alternative culture media for fungal growth using different formulation of protein sources”. Journal of Annals of Biological Research, 5 (1) (2014) 36-39.

Masaphy. “Development of Media for Growth and Enumeration of Fungi from Water”. Laboratory Protocols in Fungal Biology: Current Methods in Fungal Biology, Fungal Biology (2013).

Braaksma, M., Age, K., Smilde, Marie, Werf, V. D., dan Punt, P. J. “The effect of environmental conditions on extracellular protease activity in controlled fermentations of Aspergillus niger”. Journal of Microbiology 155, (2009) 3430–3439.

Mujahidy, J. A., “Hassan, M., Rahman, M., dan Rashid, M. Isolation and characterization of Rhizobium spp. and determination of their potency for growth factor production”. International Research Journal of Biotechnology (ISSN: 2141-5153) Vol. 4(7) (2013) 117-123.

Woomer,P. L., Karanja, N., Kisamuli, S. M., Murwira, M., dan Bala, A. “A revised manual for rhizobium methods and standard protocols available on the project website”. A revised manual for rhizobium methods and standard protocols available on the project website (2011).

Ching, H. Y., Ahmed, O. H., Kassim, S., dan Majid, N. M. A. “Co- composting of pineapple leaves and chicken manure slurry”. International Journal Of Recycling of Organic Waste in Agriculture (2013) 2:23.

Neeraj, Gaurav, S.S., Chatterjee, S.C., Sachin, dan Chandra, M. “Efficient Nitrogen Fixing Rhizobial Isolate Infecting Vigna radiata l. Asian Journal of Agricultural Sciences” 1(2): (2009) 62-65.

Maisonneuve, E. dan Gerdes, K. “Molecular mechanisms underlying bacterial persisters”. Leading Edge Review Cell (2014) 157.

Matanjun, P., Mohamed, S., Mustapha, N. M., dan Muhammad, K. “Nutrient content of tropical edible seaweeds, Eucheuma cottonii, Caulerpa lentillifera and Sargassum polycystum”. Journal of Appl Phycol (2008) 21:75–80.

Poole, P. dan Allaway, D. “Carbon and nitrogen metabolism in Rhizobium. advances in microbial physiology”. Volume (2000) 43.

Dunn, M. F. “Key roles of microsymbiont amino acid metabolism in rhizobia-legume interactions”. Crit Rev Microbiol, Early Online (2014) 1–41.

Chancharoonpong, C., huenjit, Hsieh, P. C., dan Sheu, S. C. “Enzyme production and growth of Aspergillus oryzae S. On soybean koji fermentation”. APCBEE (Procedia 2012) 000–000.

Barakah, F., Radwan, S., dan Aziz, R. “Using Biotechnology in Recy cling Agricultural Waste for Sustainable Agriculture and Environmental Protection”. Int.J.Curr.Microbiol.App.Sci 2( 12 ): (2013) 446 – 459.

Tripetchkul, S., Pundee, K., Koonsrisuk,S., dan Akeprathumchai, S. 2012. “Co-composting of coir pith and cow manure: initial C/N ratio vs physico-chemical changes”. International Journal Of Recycling of Organic Waste in Agriculture (2012) 1:15.

Sadik, M.W., El Shaer, H.M., Yakot, H. M. “Recycling of agriculture and animal farm wastes into compost using compost activator in saudi arabia”. J. Int. Environmental Application & Science, Vol. 5 (3): (2010) 397-403.


Full Text: PDF

CC Licencing


Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

Refbacks

  • There are currently no refbacks.


Lembaga Penjaminan Mutu, Pengelolaan dan Perlindungan Kekayaan Intelektual (LPMP2KI) ITS
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.