Peningkatan Masa Simpan Aktivator Kompos melalui Variasi Sumber Nitrogen

Arida Wahyu Barselia, Dr.techn.Endry Nugroho Prasetyo,MT, Endry Nugroho Prasetyo
Submission Date: 2016-01-26 20:15:45
Accepted Date: 2016-04-28 10:46:38


Kompos merupakan hasil degradasi bahan organik secara aerob maupun anaerob dengan memanfaatkan potensi mikroorganisme sebagai aktivator kompos. Kualitas aktivator kompos sangat tergantung dari kecepatan degradasi yang dimiliki dan lama masa simpannya. Medium pertumbuhan berpengaruh penting terhadap masa simpan aktivator kompos karena untuk mengomptimalkan viabilitas sel dalam waktu yang lama.

Salah satu komponen medium pertumbuhan adalah nitrogen berupa protein atau senyawa peptida yang dapat memengaruhi masa simpan (life span) aktivator kompos. Kompleksitas struktur protein menyebabkan pemanfaatan nitrogen untuk pertumbuhan juga berbeda, yang salah satunya berpengaruh terhadap masa simpan aktivator kompos.

Tujuan penelitian ini adalah mengetahui pengaruh beberapa sumber nitrogen antara lain yeast extract, pepton, susu skim, dan rumput laut, yang berpotensi untuk meningkatan masa simpan aktivator kompos.

Konsorsium aktivator kompos berupa Aspergillus niger dan Rhizobium sp. dengan perbandingan 1:1 dikultur pada medium variasi sumber nitrogen dengan konsentrasi yang berbeda yaitu 2,5% dan 1,25% dengan 3 kali pengulangan. Jumlah koloni dihitung dengan metode TPC (Total Plate Agar), Kadar protein terlarut pada medium kultur dianalisis dengan metode Bradford. Hasil kultur aktivator kompos diaplikasikan untuk pembuatan kompos. Data dianalisis secara deskriptif berupa kurva pertumbuhan koloni, kemiringan kurva pertumbuhan aktivator kompos,kadar protein terlarut, suhu kompos, dan tabel analisis rasio C/N. Hasil penelitian didapatkan bahwa medium rumput laut konsentrasi 1,25% mampu meningkatkan masa simpan aktivator kompos karena hasil kemiringan kurva pertumbuhan relatif rendah sehingga diasumsikan jumlah sel yang cenderung stabil. Sedangkan nilai rasio C/N terbaik adalah aktivator kompos pada medium rumput laut konsentrasi 1,25% karena nilai yang didapatkan sebesar 14,15/1.Sehingga medium rumput laut konsentrasi 1,25% dapat dimanfaatkan untuk meningkatkan masa simpan aktivator kompos.


aktivator kompos; masa simpan; variasi dumber nitrogen.


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