Optimasi Amobilisasi Bromelin Menggunakan Matriks Pendukung Kitosan

Maliha Sya'bana, Refdinal Nawfa
Submission Date: 2016-07-25 14:01:16
Accepted Date: 2016-11-23 18:14:55

Abstract


Optimasi amobilisasi bromelin dengan matriks pendukung kitosan telah diteliti. Enzim bromelin yang diisolasi dari buah nanas mentah maupun matang diperoleh aktivitas tertinggi pada pengendapan 40% dengan ammonium sulfat jenuh. Kondisi optimum amobilisasi enzim bromelin didapatkan pada konsentrasi kitosan 240,4 mg dan bromelin 10 mg dengan jumlah enzim teramobil  9,5417 mg dan aktivitas dalam 10 mg enzim amobil yang diuji adalah 86,2067 Unit. Uji aktivitas enzim amobil terhadap pengaruh substrat kasein didapatkan aktivitas tertinggi pada kasein 3000 ppm. Uji perulangan enzim amobil dapat digunakan hingga enam kali dengan efisiensi 57,69%.

Keywords


bromelin; amobilisasi; kitosan; kasein; aktivitas; efisiensi

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