PenggunaanAgar-agar Komersial sebagai Media Gel Elektroforesis Pada Zat Warna Remazol : Pengaruh Komposisi Buffer, pH Buffer dan Konsentrasi Media

DraIta Ulfin, Fredy Kurniawan, Atik Rohmana Maftuhatul Fuad
Submission Date: 2016-07-27 05:33:12
Accepted Date: 2016-11-23 18:14:55

Abstract


Abstrak—Elektroforesis gel menggunakan media agar-agar komersial untuk zat warna remazol  telah dilakukan. Agar-agar komersial digunakan sebagai alternatif media elektroforesis gel untuk zat warna remazoBrilliant Blue R, Red RB, Yellow FG, Turquoise Blue G dan Violet 5R dengan beberapa variabel optimasi elektroforesis gel antara lain : pengaruh komposisi buffer, pH buffer, dan konsentrasi media yang dilakukan pada tegangan 150 volt selama 15 menit. Kemudian diperoleh kondisi optimal menggunakan buffer dengan komposisi NaH2PO4.H2O + Na2HPO4. Pada pH buffer 9 didapatkan jarak migrasi terjauh untuk masing-masing zat warna RBBR, RYFG, RRRB, RTBG dan RV5R secara berturut-turut adalah : 29,11 mm; 35,40 mm; 33,40 mm; 32,07 mm; dan 31,07 mm. Pada konsentrasi 2% didapatkan jarak migrasi untuk kelima zat warna remazol paling jauh, yaitu : RBBR 29,13 mm; RYFG 34,14 mm; RRRB 31,16 mm; RTBG 30,25 mm dan RV5R 29,20 mm.

Keywords


elektroforesis gel; zat warna remazol; agar-agar komersial

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