Pemanfaatan Nata de Coco sebagai Media Gel Elektroforesis Pada Zat Warna Remazol : Pengaruh pH, Waktu dan Aplikasi pemisahan gelatin

Lutfi Andre Yahya, Ita Ulfin, Fredy Kurniawan
Submission Date: 2016-07-28 14:37:27
Accepted Date: 2016-11-23 18:14:55

Abstract


Nata de coco telah digunakan sebagai pengganti media gel elektroforesis pada sampel pewarna remazol.  Pada penelitian ini, diamati pengaruh pH larutan buffer, waktu elektroforesis dan aplikasi pemisahan gelatin menggunakan elektroforesis dengan media nata de coco. Nata de coco yang berumur 4 hari memiliki ketebalan yang sesuai yaitu 0,454 cm dan waktu inkubasi yang tidak terlalu lama sehingga umur tersebut digunakan dalam penelitian ini. Hasil resolusi elektroforesis terbaik didapatkan pada pengggunaan buffer fosfat yang terbuat dari garam–garamnya. Pada pengaruh pH dinyatakan dengan kekuatan ion, dimana semakin besar kekuatan ion larutan, maka semakin jauh jarak migrasi remazol. Lama waktu elektroforesis mempengaruhi jarak migrasi remazol Kondisi optimum elektroforesis gel menggunakan nata yang didapat, digunakan untuk uji pemisahan gelatin menggunakan pewarna remazol turquoise dan didapatkan dua pita pemisahan dengan jarak pita masing-masing 2 cm dan 3,6 cm.


Keywords


Nata de coco; Gel Elektroforesis; Remazol; Buffer fosfat; Gelatin

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