Isolasi Senyawa Antioksidan dari Kulit Batang Sonneratia ovata Backer

Fatati Nurmalasari, Taslim Ersam, Sri Fatmawati
Submission Date: 2016-08-01 09:41:07
Accepted Date: 2016-11-23 18:14:55

Abstract


Campuran Senyawa β-sitosterol (1a) dan stigmasterol (1b) telah diisolasi dari ekstrak metanol kulit batang Sonneratia ovata Backer dengan titik leleh 127-128 oC. Sonneratia ovata Backer merupakan tumbuhan mangrove yang berasal dari Pulau Dobo, Kepulauan Aru, Maluku yang telah banyak digunakan sebagai obat tradisional untuk berbagai penyakit. Isolasi dilakukan dengan ekstraksi (maserasi) dan kromatografi cair vakum (KCV). Senyawa hasil isolasi dikarakterisasi dengan IR dan 13C-NMR. Fraksi dan senyawa hasil isolasi menunjukkan aktivitas antioksidan dengan uji radikal DPPH. Fraksi dan senyawa hasil isolasi menunjukkan aktivitas antioksidan yang lebih rendah dibandingkan dengan asam galat sebagai kontrol positif dengan persentase penghambatan sebesar 43,652 ± 0,013 % dan 27,159 ± 0,014% pada konsentrasi 319,458 µg/mL.

Keywords


Sonneratia; isolasi; antioksidan; DPPH

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