Pertumbuhan Kalus Jeruk JC (Japansche Citroen) pada Media Murashige and Skoog dengan Berbagai Konsentrasi NaCl

Ika Dian Puspitasari, Wirdhatul Muslihatin, Dita Agisimanto
Submission Date: 2017-07-24 12:04:41
Accepted Date: 2017-12-31 15:38:19

Abstract


Jeruk JC (Japansche Citroen) merupakan salah satu jenis jeruk yang sering digunakan sebagai stok batang bawah. Batang bawah harus mampu tumbuh dan beradaptasi pada kondisi lingkungan yang kurang baik, salah satunya terhadap cekaman salinitas. Tujuan penelitian ini yaitu untuk mengetahui pengaruh media dengan konsentrasi NaCl yang berbeda terhadap pertumbuhan kalus jeruk JC (Japansche Citroen) secara in vitro. Eksplan berupa kalus jeruk JC diinokulasikan pada media MS cair dengan penambahan NaCl konsentrasi 0 mM, 50 mM, 100 mM, dan 150 mM. Parameter pengamatan yang digunakan yaitu berat basah kalus. Data dianalisis menggunakan ANOVA One Way dan dilanjutkan dengan uji Tukey pada taraf kepercayaan 95%. Berdasarkan hasil penelitian ini konsentrasi NaCl berpengaruh nyata terhadap pertumbuhan kalus jeruk JC. Pada konsentrasi 150 mM kalus jeruk JC dapat bertahan hidup terhadap kondisi salinitas tinggi. Berat basah kalus tertinggi yaitu pada perlakuan kontrol sebesar 0,876 g, sedangkan berat basah terendah yaitu sebesar 0,573 g pada perlakuan NaCl konsentrasi 150 mM.

 


Keywords


Japansche Citroen; Kalus batang bawah; NaCl; Pertumbuhan kalus

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