Induksi Perakaran Teh (Camellia sinensis L.) Secara In Vitro pada Klon yang Berbeda

Fitriana Ilma Farida, Wirdhatul Muslihatin
Submission Date: 2017-08-01 00:18:14
Accepted Date: 2017-12-31 15:38:20

Abstract


Abstrak— Pembentukan akar teh (Camellia sinensis L.) merupakan tahapan penting secara in vitro. Inisiasi perakaran tanaman dapat dipacu dengan menambahkan Zat Pengatur Tumbuh (ZPT). Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi ZPT untuk inisiasi akar tanaman teh secara in vitro pada klon teh yang berbeda. Klon yang digunakan adalah Tea Research of Sri Lanka (TRI) 2024 dan TRI 2025. Eksplan berupa daun, diinokulasikan pada media induksi kalus dengan penambahan BAP 2 mg/l dan NAA 3 mg/l. Kalus yang terbentuk disubkultur pada media induksi tunas dengan penambahan BAP 3 mg/l, selanjutnya, disubkultur ke dalam media perakaran dengan penambahan IBA (Indole Butyric Acid) 0, 1, 2, 3 mg/l. Hasil penelitian menunjukkan, pada media perakaran tidak terbentuk akar, tetapi terbentuk embrio somatik dengan tahap yang berbeda, yaitu tahap globular, hati dan torpedo. Embrio somatik yang terbentuk memiliki perbedaan warna yaitu, hijau kekuningan, hijau dan hijau kecoklatan. Selain itu, mempunyai struktur: remah, intermediet dan kompak, serta mempunyai berat basah embrio somatik sebesar 10-290 mg.


Keywords


Camellia sinensis L.; IBA Perakaran; Teh; TRI 2024; TRI 2025

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