Pengaruh Mutagen Kimia EMS (Ethyl Methane Sulphonate) Terhadap Daya Berkecambah Benih Tanaman Tembakau var. Marakot

Bangun Surya Putra, Kristanti Indah Purwani
Submission Date: 2017-08-01 12:32:32
Accepted Date: 2017-12-31 15:38:20

Abstract


Penyediaan benih bermutu merupakan permasalahan dalam peningkatan produksi tembakau. Induksi mutasi merupakan salah satu cara untuk mendapatkan varietas unggul yang memiliki produktivitas tinggi dan kualitas yang baik. Beberapa penelitian mengenai penggunaan mutagen kimia EMS (Ethyl Methane Sulphonate) menunjukkan pengaruh terhadap perkecambahan benih tanaman sehingga diperlukan penelitian untuk mengetahui pengaruh konsentrasi mutagen EMS terhadap daya berkecambah benih tanaman tembakau. Beberapa variasi konsentrasi EMS yang digunakan pada penelitian ini yaitu 0,1%, 0,5%, 1%, dan 1,5%. Hasil penelitian menunjukkan bahwa perbedaan konsentrasi mutagen EMS berpengaruh nyata terhadap daya berkecambah benih tembakau. Perlakuan EMS dengan konsentrasi 1% dan 1,5% menunjukkan penurunan daya kecambah benih.

Keywords


Benih tembakau; Daya kecambah; Induksi mutasi; Mutagen EMS

References


R. Hanadyo., T. Hadiastono., dan M. Martosudiro. Pengaruh Pemberian Pupuk Daun Cair Terhadap Intensitas Serangan Tobacco Mosaic Virus (TMV), Pertumbuhan, dan Produksi Tanaman Tembakau (Nicotiana tabacum L.). Jurnal HPT. 1(2): 28-36 (2013).

E. A. Putri., A. Suwandari., dan J. A. Ridjal. Analisis Pendapatan dan Efisiensi Biaya Usahatani Tembakau Maesan 2 di Kabupaten Bondowoso. JSEP. 8(1): 64-69 (2015).

M. K. Lesilolo., J. Patty dan N. Tetty. Penggunaan Desikan Abu dan Lama Simpan Terhadap Kualitas Benih Jagung (Zea mays L.) pada Penyimpanan Ruang Terbuka. Jurnal Agrologia. 1(1): 51-59 (2012).

A. H. Rachman. Status Pertembakauan Nasional, Prosiding Lokakarya Nasional Agribisnis Tembakau. Pusat Penelitian dan Pengembangan Perkebunan. Badan Penelitian dan Pengembangan Pertanian, Surabaya (2007).

M. Hasanah. Peran Mutu Fisiologik Benih dan Pengembangan Benih Tanaman Industri. Jurnal Litbang Pertanian. 21(2): 8 (2002).

SNI. Standar Nasional Indonesia: Benih Tembakau Kelas Benih Dasar dan Benih Sebar. SNI. 01-7161-2006. ICS 27-180. Standar Nasional Indonesia. Badan Standarisasi Nasional (2006).

L.Sari., A. Purwito., D. Sopandie., R. Purmaningsih., dan Enny Sudarmonowati. Pengaruh Irradiasi Sinar Gamma pada Pertumbuhan Kalus dan Tunas Tanaman Gandum (Triticum aestivum L.). Jurnal Ilmu Pertanian. 1(1) : 44-50 (2015).

L.V. Crowder. Genetika Tumbuhan. Terjemahan Kusdiarti, L., Sutarso (ed). Gadjah Mada University Press, Yogyakarta (1993).

A.M. Van Harten. Mutation Breeding: Theory and Practical Application. Cambridge University Press, New York. (1998).

M. Imelda., Deswina, P., Hartati, S., Estiati, A., and Atmowijoyo, S. Chemical Mutation by Ethyl Methane Sulfonate (EMS) for Bunchy Top. Virus Resistence in Banana. Annales Bogorienses. 38(3): 205-211 (2000).

R.D. Purwati., Budi, U., dan Sudarsono, S. Penggunaan Asam Fusarat dalam Seleksi in vitro untuk Resistensi terhadap Fusarium oxysporum. Jurnal Littri. 7(2): 80-91 (2007).

R.D. Purwati., Sudjindro, K.E., dan Sudarsono, S. Keragaman Genetika Varian Abaka yang Diinduksi dengan Ethyl Methane Sulphonate (EMS). Jurnal Littri. 15(4): 152-161 (2008).

N. Jabeen., and Mirza, B. Ethyl Methane Sulfonate Induces Morphological Mutations in Capsicum annuum. International Journal of Agriculture Biology. 6(2): 340-345 (2004).

A.B. Talebi., A.B. Talebi., and Behzad Shahrokhifar. Ethyl Methane Sulphonate (EMS) Induced Mutagenesis in Malaysian Rice (cv. MR219) for Lethal Dose Determination. American Journal of Plant Sciences. 3: 1661-1665 (2012).

H. Kuswanto. Dasar-Dasar Teknologi Produksi dan Sertifikasi Benih. Penerbit Andi, Yogyakarta (1996).

S. Amarnath and A.B. Prasad. Induced Variability in Homozygous and Heterozygous Genotypes of Tobacco. Indian Journal of Genetics. 58(1): 69-77 (1998).

D. Dhakshanamoorthy., R. Selvaraj., dan A. Chidambaram. Physical and Chemical Mutagenesis in Jatropha Curcas L. to Induce Variability in Seed Germination, Growth and Yield Traits. Journal of Plant Biology. 55(2): 113–125 (2012).

J. Sambrook, and D.W. Russel. Molecular Cloning : A Laboratory Manual. Eds 3. New York : Cold Spring Harbor Laboratory Press (2001).

S. Jayakumar., and Selvaraj R. Mutagenic Effectiveness and Efficiency of Gamma Rays and Ethyl Methane Sulphonate in Sunflower (Helianthus annus L.). Madras Jurnal Agriculture. 90(1): 574-576 (2003).

A. R. Loveless. Prinsip Prinsip Biologi Tumbuhan Untuk Daerah Tropik. Jilid 1. Jakarta: PT. Gramedia Pustaka (1991).

R. Singh., and Kole, C.R. Effect of Mutagenic Treatments with EMS on Germination and Some Seedling Parameters in Mungbean. Crop Research. 30(2): 236-240 (2005).

F. Al-Qurainy., and S. Khan. Mutagenic Effects of Sodium Azide and its Application in Crop Improvement. World Applied Sciences Journal. 7(2): 220-226. (2009).

N. K. Rustini., dan M. Pharmawati. Aksi Ethyl Methane Sulphonate terhadap Munculnya Bibit dan Pertumbuhan Cabai Rawit (Capsicum frutescens L.). Jurnal Bioslogos. 4(1):1-8 (2013).

D. K. Lee., Y. S. Kim., and J. K. Kim. Determination of The Optimal Condition for Ethylmethane Sulfonate-Mediated Mutagenesis in Korean Commercial Rice, Japonica Cv. Dongjin. Applied Biological Chemistry (2017).

Priyono dan A.W Susilo. Respon Regenerasi In vitro Eksplant Sisik Mikro Kerk Lily (Lilium longiflorum) terhadap Ethyl Methane Sulfonate (EMS). Jurnal Ilmu Dasar. 3(2): 74-79 (2002).


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