Aktivitas Enzim Endoglukanase (EG) dan Lignin Peroksidase dari Penicillium sp. Pada Media Pertumbuhan Bekatul dan Tongkol Jagung

Arzulinda Maulidar Rahmasyitha, Enny Zulaika, Kuswytasari Kuswytasari
Submission Date: 2018-08-03 16:25:25
Accepted Date: 2019-03-29 06:57:02

Abstract


Bekatul dan tongkol jagung memiliki kandungan lignin dan selulosa yang tersusun oleh unsur nitrogen dan karbon. Sehingga bekatul dan tongkol jagung dapat digunakan sebagai media pertumbuhan fungi. Beberapa spesies jamur menghasilkan enzim endoglukanase (EG) dan lignin peroksidase (LiP). Penelitian ini bertujuan untuk mengetahui aktivitas enzim endoglucanase (EG) dan lignin peroksidase (LiP) pada Penicillium sp. dengan media pertumbuhan bekatul dan tongkol jagung. Aktivitas enzim endoglukanase (EG) diukur menggunakan metode DNS, sedangkan aktivitas enzim lignin peroksidase (LiP) diukur dengan metode oksidasi. Hasil penelitian menunjukkan bahwa aktivitas enzim endoglukanase (EG) dan LiP pada Penicillium sp. yang tertinggi pada media bekatul adalah sebesar 0,135 U/ml dan 32,131 U/ml. Pada media tongkol jagung aktivitas enzim endoglukanase (EG) dan LiP tertinggi adalah sebesar 0,104 U/ml dan 26,695 U/ml.


Keywords


Bekatul; lignoselulosa; Pennicillium sp.; tongkol jagung.

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