Pengaruh Penambahan Kombinasi Konsentrasi ZPT NAA dan BAP terhadap Pertumbuhan dan Perkembangan Biji Dendrobium Taurulinum J.J Smith Secara In Vitro
Submission Date: 2012-07-27 19:50:09
Accepted Date: 2012-09-11 00:00:00
Abstract
Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi konsentrasi zat pengatur tumbuh NAA dan BAP yang efektif untuk perkembangan biji D. taurulinum secara in vitro. Kombinasi konsentrasi zat pengatur tumbuh NAA yang dipakai adalah (0,1; 0,2; 0,3; 0,4; 0,5 mg/L) dan BAP (0,1; 0,3; 0,5 mg/L) dengan perlakuan tanpa penambahan Zat Pengatur Tumbuh sebagai kontrol. Penelitian menunjukkan bahwa setelah 5 bulan inokulasi hasil terbaik ditunjukkan pada medium tanpa penambahan ZPT dengan 100% biji berkembang menjadi planlet. Pada penambahan berbagai kombinasi Zat Pengatur Tumbuh didapatkan hasil dominasi pertumbuhan hanya mampu membentuk protocorm. Hal ini membuktikan bahwa suatu organ dan jaringan tumbuhan mengandung hormon endogen yang dapat mempengaruhi pertumbuhan dan perkembangan organ atau jaringan tersebut hingga tahapan yang paling sempurna walaupun tidak ditambahkan zat pengatur tumbuh dari luar
Keywords
BAP; In Vitro; NAA; pertumbuhan biji D. taurulinum; Zat Pengatur Tumbuh
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