Potensi Isolat Bakteri Pseudomonas Sebagai Pendegradasi Plastik
Submission Date: 2015-08-06 17:46:44
Accepted Date: 2016-01-20 16:26:13
Abstract
Plastik adalah salah satu polimer sintetik atau buatan manusia yang merupakan rantai panjang molekul polimer. Plastik yang terakumulasi menyebabkan pencemaran lingkungan. Salah satu solusi untuk menangani adanya masalah lingkungan ini adalah dengan biodegradasi dengan menggunakan mikroorganisme. Tujuan penelitian ini adalah untuk mengetahui kemampuan isolat Pseudomonas L1 dalam mendegradasi plastik. Plastik warna hitam, putih dan transparan dengan ukuran 10x3 cm diinkubasi selama 3 bulan didalam botol uji yang berisi 300 gram plastik steril, 315 medium MSM dan 35 ml isolat Pseudomonas L1. Biodegradasi plastik dihitung berdasarkan selisih berat kering sebelum dan setelah 3 bulan masa inkubasi dengan interval waktu pengukuran setiap 3 minggu sekali. Isolat Pseudomonas L1 terbukti mampu mendegradasi plastik hitam dengan rata-rata degradasi sebesar 2,7%, plastik putih sebesar 3,3% dan plastik transparan sebesar 4,5% selama 3 bulan masa inkubasi. Kerapatan sel isolat Pseudomonas L1 uji terdeteksi dengan mengukur kekeruhan isolat pada OD dengan panjang gelombang 600 nm. Isolat Pseudomonas L1 lebih cenderung membentuk biofilm dibandingkan hidup bebas pada kolom air. Kepadatan sel pada biofilm plastik hitam relatif lebih tinggi daripada 2 plastik yang lain. Pada plastik hitam OD biofilm yang terdeteksi dengan panjang gelombang 600 nm sebesar 0,038, sedangkan pada plastik putih sebesar 0,026 dan plastik transparan sebesar 0,029.
Keywords
Biodegradasi, Plastik, Pseudomonas.
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