Pengaruh pH dan Suhu Terhadap Aktivitas Protease Penicillium sp.

Abstract

Tujuan penelitian ini adalah untuk mengetahui pengaruh pH dan suhu terhadap aktivitas protease pada Penicillium sp.3 T₃f₂. Selanjutnya, isolat Penicillium sp.di kultur dalam media produksi protease untuk menghasilkan protease. Suhu yang digunakan adalah 30⁰ – 50⁰C sedangkan pH-nya 4 – 8. Aktivitas protease ditentukan dan diukur dengan spektrofotometer pada panjang gelombang 275 nm, dengan kasein sebagai substrat. Berdasarkan uji ANOVA yang dilanjutkan dengan uji Duncan dengan taraf kepercayaan 95%, didapatkan bahwa hanya pH yang berpengaruh terhadap aktivitas protease. Aktivitas protease Penicillium sp. tertinggisebesar 2.416 U/ml, dan dicapai pada pH 8 dan suhu 40⁰C.

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