Bakteri Tanah Sampah Pendegradasi Plastik dalam Kolom Winogradsky

Abstract

Penggunaan plastik berupa kantong kresek hasil daur ulang dengan berbagai warna sangat diminati oleh masyarakat. Sifat plastik yang tidak mudah terdegradasi di alam mengakibatkan masalah lingkungan. Penelitian ini dilakukan untuk mengisolasi dan mengkarakterisasi bakteri tanah sampah yang mampu mendegradasi plastik secara biokimia. Parameter biodegradasi plastik yang diukur adalah prosentase kehilangan berat kering, pengukuran densitas sel biofilm, densitas sel kolom air dan pH tiap bulan selama 4 bulan masa inkubasi. Dari penelitian didapatkan persentase kehilangan berat kerig plastk hitam lebih tinggi daripada plastik putih bening. Hasil yang diperoleh menunjukkan bahwa isolat bakteri tanah sampah pendegradasi plastik yaitu Gram positif basil (PPs 2, PPs 7, PPs 9, dan PPs 11) dan Gram negatif basil (PPs 1, PPs 4, PPs 5, PPs 6, PPs 8, PPs 10, PPs 12 dan PPs 13 )dan hanya PPs 3 termasuk Gram negatif kokus.

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